3NZ4
Crystal Structure of a Taxus Phenylalanine Aminomutase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0782 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.733, 76.113, 120.392 |
| Unit cell angles | 90.00, 120.43, 90.00 |
Refinement procedure
| Resolution | 103.810 - 2.380 |
| R-factor | 0.1887 |
| Rwork | 0.183 |
| R-free | 0.23926 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.310 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.810 | 2.420 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Number of reflections | 49662 | |
| Completeness [%] | 96.8 | 79.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.1M HEPES, pH 7.0, 1.0M LiCl, 15% PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
