3NYB
Structure and function of the polymerase core of TRAMP, a RNA surveillance complex
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-04-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.28215 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 92.720, 92.720, 103.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.656 - 2.701 |
| R-factor | 0.1982 |
| Rwork | 0.194 |
| R-free | 0.25810 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.034 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.800 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.690 | 2.600 |
| Rmerge | 0.077 | 0.109 | 0.082 |
| Number of reflections | 16163 | ||
| <I/σ(I)> | 4.3 | 2.3 | |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 277 | 100 mM sodium citrate at pH 5.8, 200 mM sodium acetate, 11% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
