3NY2
Structure of the ubr-box of UBR2 ubiquitin ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9779 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.390, 61.456, 72.806 |
| Unit cell angles | 65.05, 89.98, 90.01 |
Refinement procedure
| Resolution | 50.000 - 2.610 |
| R-factor | 0.23265 |
| Rwork | 0.230 |
| R-free | 0.28808 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.094 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 12503 | |
| Completeness [%] | 94.8 | 79.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.96M sodium citrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
