3NY1
Structure of the ubr-box of the UBR1 ubiquitin ligase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-28 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.2836 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 29.702, 49.262, 43.831 |
Unit cell angles | 90.00, 100.51, 90.00 |
Refinement procedure
Resolution | 43.100 - 2.085 |
R-factor | 0.19673 |
Rwork | 0.194 |
R-free | 0.24783 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.471 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.080 | 2.080 |
Number of reflections | 7043 | |
Completeness [%] | 98.7 | 86.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Bis-Tris, 25% PEG3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |