3NXL
Crystal structure of Glucarate dehydratase from Burkholderia cepacia complexed with magnesium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-29 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97915 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 165.289, 121.452, 108.652 |
Unit cell angles | 90.00, 92.60, 90.00 |
Refinement procedure
Resolution | 34.235 - 1.885 |
R-factor | 0.1917 |
Rwork | 0.190 |
R-free | 0.22070 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.984 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 34.235 |
High resolution limit [Å] | 1.885 |
Number of reflections | 167926 |
Completeness [%] | 97.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 3350,0.2M sodium malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |