3NWZ
Crystal Structure of BH2602 protein from Bacillus halodurans with CoA, Northeast Structural Genomics Consortium Target BhR199
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97901 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.243, 89.733, 71.850 |
| Unit cell angles | 90.00, 107.37, 90.00 |
Refinement procedure
| Resolution | 27.883 - 2.566 |
| R-factor | 0.2326 |
| Rwork | 0.231 |
| R-free | 0.26390 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.331 |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.080 |
| Number of reflections | 38995 |
| <I/σ(I)> | 18 |
| Completeness [%] | 99.1 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution: 0.2M ammonium sulfate, 0.1M hepes, 18% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
