3NW8
Glycoprotein B from Herpes simplex virus type 1, Y179S mutant, high-pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 3 |
| Unit cell lengths | 117.985, 117.985, 321.537 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.690 - 2.759 |
| R-factor | 0.2037 |
| Rwork | 0.202 |
| R-free | 0.24250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nwa |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.601 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.690 | 2.810 |
| High resolution limit [Å] | 2.759 | 2.760 |
| Rmerge | 0.115 | |
| Number of reflections | 118749 | |
| <I/σ(I)> | 12.9 | 1.38 |
| Completeness [%] | 92.1 | 83 |
| Redundancy | 4.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 10% PEG 4000, 0.2M NaCl, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
