3NVQ
Molecular mechanism of guidance cue recognition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-10-10 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 99.645, 126.078, 236.419 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.400 - 2.400 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.27700 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.049 | 0.468 |
| Number of reflections | 114947 | |
| <I/σ(I)> | 29.8 | 2.1 |
| Completeness [%] | 98.6 | 86.8 |
| Redundancy | 4.3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 12% PEG1000, 0.1M Tris pH7.5, 0.2M Li2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
