3NVO

The Soluble Domain Structure of the ZntB Zn2+ Efflux System

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	45.039, 93.845, 68.231
Unit cell angles	90.00, 91.58, 90.00

Refinement procedure

Resolution	38.658 - 2.300
R-factor	0.204
Rwork	0.201
R-free	0.25610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.834
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.390
High resolution limit [Å]	2.300	2.300
Number of reflections	24331
Completeness [%]	96.0	74.4
Redundancy	3.5	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1