3NVM

Structural basis for substrate placement by an archaeal box C/D ribonucleoprotein particle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MAR scanner 300 mm plate
Spacegroup name	P 31 1 2
Unit cell lengths	100.623, 100.623, 265.355
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.431 - 3.408
R-factor	0.2455
Rwork	0.242
R-free	0.31600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.264
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	3.400	3.400
Number of reflections	32901
Completeness [%]	80.0	26

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	303	0.1 M imidazole pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 303K
1	VAPOR DIFFUSION, HANGING DROP	6.4	303	0.1 M imidazole pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 303K