3NVJ
Crystal structure of the C143A/C166A mutant of Ero1p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-05-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.514 |
| Spacegroup name | P 62 |
| Unit cell lengths | 107.220, 107.220, 124.227 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.200 |
| R-factor | 0.251 |
| Rwork | 0.244 |
| R-free | 0.29980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rq1 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 13131 |
| Completeness [%] | 97.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
