3NVF
IIHFGS segment 138-143 from human prion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-01 |
| Detector | MAR CCD 225 |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 27.546, 31.017, 4.804 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.600 - 1.800 |
| R-factor | 0.2133 |
| Rwork | 0.212 |
| R-free | 0.22630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.657 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 1.940 |
| High resolution limit [Å] | 1.800 | 3.080 | 1.800 |
| Rmerge | 0.216 | 0.197 | 0.395 |
| Number of reflections | 443 | ||
| <I/σ(I)> | 6.8 | ||
| Completeness [%] | 85.4 | 94.5 | 67 |
| Redundancy | 3.8 | 5.1 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 100mM Tris pH 7.4, 25% MPD, vapor diffusion, hanging drop, temperature 298K |
