3NVE
MMHFGN segment 138-143 from Syrian Hamster prion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-01-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 9.513, 11.784, 36.541 |
| Unit cell angles | 90.00, 93.04, 90.00 |
Refinement procedure
| Resolution | 36.490 - 1.700 |
| R-factor | 0.206 |
| Rwork | 0.196 |
| R-free | 0.26880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.904 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.3) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 80.000 | 80.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 3.662 | 1.700 |
| Rmerge | 0.162 | 0.163 | 0.150 |
| Number of reflections | 939 | ||
| <I/σ(I)> | 15.9 | ||
| Completeness [%] | 97.2 | 99.1 | 88 |
| Redundancy | 10.5 | 10.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.2 M Ammonium sulfate, 200 mM BisTris pH 7.5, vapor diffusion, hanging drop, temperature 298K |
