3NV0
Crystal structure and mutational analysis of the NXF2/NXT1 heterodimeric complex from caenorhabditis elegans at 1.84 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH - SAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Detector technology | CCD |
| Collection date | 2008-08-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.004, 49.547, 148.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.010 - 1.840 |
| R-factor | 0.167 |
| Rwork | 0.166 |
| R-free | 0.19100 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.585 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol ((PHENIX.AUTOSOL)) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.870 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.068 | 0.384 |
| Number of reflections | 27423 | |
| <I/σ(I)> | 13.3 | 3.3 |
| Completeness [%] | 98.9 | 98.1 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | PEG 3350, HEPES PH 7.0, NACL, KCL, BME (2-MERCAPTOETHANOL), VAPOR DIFFUSION, SITTING DROP, temperature 293K |
