3NTT

Structural insights of Adeno-Associated virus 5. A gene therapy Vector for Cystic Fibrosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	264.700, 447.900, 629.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.959 - 3.450
R-factor	0.2515
Rwork	0.252
R-free	0.25150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g8g
RMSD bond length	0.005
RMSD bond angle	1.015
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.570
High resolution limit [Å]	3.450	3.450
Number of reflections	762314
<I/σ(I)>	7.9
Completeness [%]	78.6	58.5
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	20mM Tris HCL, 350mM NaCl, MgCl2, 1.5% PEK 8K, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	20mM Tris HCL, 350mM NaCl, MgCl2, 1.5% PEK 8K, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K