3NTE

Crystal Structure of the Wild-type Full-Length HIV-1 Capsid Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-24
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	47.910, 86.532, 55.589
Unit cell angles	90.00, 99.73, 90.00

Refinement procedure

Resolution	29.048 - 1.950
R-factor	0.2064
Rwork	0.204
R-free	0.25340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 1ak4 1a43
RMSD bond length	0.008
RMSD bond angle	1.093
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.6.1_336)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.020
High resolution limit [Å]	1.950	4.200	1.950
Rmerge	0.064	0.040	0.501
Number of reflections	31847
<I/σ(I)>	12.5
Completeness [%]	97.2	99.6	82.4
Redundancy	3.7	3.7	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	9-12% PEG 3350, 0.2 M sodium iodide, 0.1 M Bis-Tris-propane pH 6.5, 0.01 M iron(III) chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K