3NT9
CRYSTAL STRUCTURE OF LSSmKate1 red fluorescent proteins with large Stokes shift
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.08 |
Spacegroup name | P 43 |
Unit cell lengths | 72.177, 72.177, 226.562 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.810 - 1.990 |
R-factor | 0.18463 |
Rwork | 0.182 |
R-free | 0.23179 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.835 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.2) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.020 |
High resolution limit [Å] | 1.990 | 5.400 | 1.990 |
Rmerge | 0.075 | 0.034 | 0.717 |
Number of reflections | 76485 | ||
<I/σ(I)> | 13.4 | ||
Completeness [%] | 96.7 | 99.5 | 66.8 |
Redundancy | 6.7 | 7.5 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 20% PEG 1000, 0.1 M phosphate-citrate, pH 5.6, 0.2 M Li2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |