3NT7
Crystal Structure of Vaccinia Virus Uracil DNA Glycosylase R187V Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 85.195, 85.195, 139.439 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.22443 |
| Rwork | 0.222 |
| R-free | 0.27402 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2owq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.118 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 6.440 | 2.400 |
| Rmerge | 0.056 | 0.027 | 0.340 |
| Number of reflections | 21064 | ||
| <I/σ(I)> | 20.1 | ||
| Completeness [%] | 89.7 | 79.2 | 94 |
| Redundancy | 5.5 | 5.6 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 1.5M Ammonium sulfate, 0.1M Hepes , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
