3NSL
Crystal Structure of S100A3 C30A+C68A double mutant expressed in insect cell
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-05-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 |
| Unit cell lengths | 101.357, 101.357, 45.209 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.350 - 1.500 |
| R-factor | 0.16182 |
| Rwork | 0.160 |
| R-free | 0.19655 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nsi |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.970 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 83178 | |
| Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | ammonium sulfate, 0.1M HEPES, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
