3NS5

Crystal structure of the RNA recognition motif of yeast eIF3b residues 76-161

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Wavelength(s)	1.5417
Spacegroup name	P 21 21 21
Unit cell lengths	30.280, 68.140, 81.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.456 - 2.598
R-factor	0.2274
Rwork	0.225
R-free	0.27250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ns6
RMSD bond length	0.001
RMSD bond angle	0.480
Data scaling software	SCALA
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.160	34.070	2.730
High resolution limit [Å]	2.598	8.200	2.590
Rmerge		0.031	0.320
Total number of observations		795	3276
Number of reflections	5632
<I/σ(I)>	10.8	19.7	2.4
Completeness [%]	99.1	98.8	94.6
Redundancy	4.4	3.7	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6		33% PEG4000 and 0.1 M Na-citrate (pH 5.6) in a sitting drop plate at 20 degrees. 1uL of protein at the concentration of 17 mg/mL was mixed with 1 uL of reservoir, VAPOR DIFFUSION, SITTING DROP