3NRX
Insights into anti-parallel microtubule crosslinking by PRC1, a conserved non-motor microtubule binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2009-04-21 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 32 |
| Unit cell lengths | 49.078, 49.078, 94.784 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.750 |
| R-factor | 0.202 |
| Rwork | 0.198 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nry |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.030 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.053 | 0.361 |
| Number of reflections | 24182 | |
| <I/σ(I)> | 31.4 | 2.62 |
| Completeness [%] | 94.0 | 94.8 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.5 | 277 | 1:1 v/v of protein ( 50 mg/ml in 80 mM PIPES, pH6.8, 1 mM MgCl2, 1 mM EDTA and 150 mM KCl) and reservoir buffer (100 mM CHES, pH 9.5, 30% PEG3000), VAPOR DIFFUSION, HANGING DROP, temperature 277K |
