3NR9
Structure of human CDC2-like kinase 2 (CLK2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-03-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 97.678, 97.678, 223.029 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.840 - 2.890 |
| R-factor | 0.19686 |
| Rwork | 0.194 |
| R-free | 0.25193 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eu9 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.427 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.900 | 3.040 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.173 | 0.989 |
| Number of reflections | 28133 | |
| <I/σ(I)> | 8.5 | 2 |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.3 | 277.15 | 25% MPD, 0.1M Bicine, pH 9.3, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
