3NR8
Crystal structure of human SHIP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-22 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.796, 61.177, 114.320 |
| Unit cell angles | 90.00, 91.90, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.21644 |
| Rwork | 0.214 |
| R-free | 0.27247 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i9y |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.146 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.770 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.152 | 0.720 |
| Number of reflections | 15338 | |
| <I/σ(I)> | 7.9 | 2 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 20% PEG 6000, 0.1M citric acid pH 5, 2mM biphenyl 2,3',4,5',6-pentakisphosphate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
