3NR7
Crystal structure of S. typhimurium H-NS 1-83
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 |
| Unit cell lengths | 130.397, 130.397, 55.061 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.600 - 3.700 |
| R-factor | 0.27366 |
| Rwork | 0.271 |
| R-free | 0.30375 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ov9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.551 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.600 | 3.830 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.100 | 0.440 |
| Number of reflections | 5676 | |
| <I/σ(I)> | 6.7 | 1.8 |
| Completeness [%] | 96.9 | 96.4 |
| Redundancy | 2.7 | 2.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.2 | 290 | 20 % (v/v) MPD, 20 mM phenol, 100 mM MES, pH 6.2, MICRODIALYSIS, temperature 290K |
