3NR1
A metazoan ortholog of SpoT hydrolyzes ppGpp and plays a role in starvation responses
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2007-09-12 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97953 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.900, 62.400, 53.900 |
Unit cell angles | 90.00, 95.30, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
Rwork | 0.203 |
R-free | 0.23020 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.032 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.13) |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.077 | 0.051 | 0.354 |
Number of reflections | 28330 | ||
<I/σ(I)> | 8.9 | ||
Completeness [%] | 99.8 | 99.2 | 99.5 |
Redundancy | 3.7 | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | 20-25% PEG 3350, 0.2M sodium citrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | 20-25% PEG 3350, 0.2M sodium citrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |