3NQK

Crystal structure of a STRUCTURAL GENOMICS, UNKNOWN FUNCTION (BACOVA_03322) from Bacteroides ovatus at 2.61 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-07
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97937,0.97908
Spacegroup name	P 3 2 1
Unit cell lengths	124.073, 124.073, 66.888
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.801 - 2.610
R-factor	0.2
Rwork	0.198
R-free	0.23600
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	0.917
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.15)
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.801
High resolution limit [Å]	2.610
Number of reflections	18340
Completeness [%]	99.9
Redundancy	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	277	0.200000000M (NH4)2Tartrate, 20.000000000% PEG-3350, No Buffer pH 6.6, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K