3NPH
Crystal structure of the pfam00427 domain from Synechocystis sp. PCC 6803
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-07 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.498 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 55.675, 55.675, 79.187 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.772 - 1.849 |
| R-factor | 0.204 |
| Rwork | 0.203 |
| R-free | 0.23430 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.988 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.430 | 1.910 |
| High resolution limit [Å] | 1.849 | 1.849 |
| Number of reflections | 11164 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 12% PEG 3000, 0.1M Bis-Tris buffer (pH 6.5), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
