3NP5
Crystal structure of an abridged form of the mature ectodomain of the human receptor-type protein tyrosine phosphatase ICA512/IA-2 AT pH 4.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-01 |
| Detector | ADSC QUANTUM 270 |
| Spacegroup name | P 41 |
| Unit cell lengths | 44.662, 44.662, 168.665 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.840 - 1.800 |
| Rwork | 0.161 |
| R-free | 0.23852 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qt7 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.968 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.042 | 0.226 |
| Number of reflections | 28186 | |
| <I/σ(I)> | 40.6 | 3.5 |
| Completeness [%] | 91.9 | 73.6 |
| Redundancy | 5.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 292 | 30% (W/V) PEG 4000, 0.1 M AcNa/AcH, PH 4.50.2 M CALCIUM CHLORIDE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 292K |
