3NOW

UNC-45 from Drosophila melanogaster

Experimental procedure

Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00000
Spacegroup name	P 2 3
Unit cell lengths	184.079, 184.079, 184.079
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.197 - 2.992
R-factor	0.2024
Rwork	0.201
R-free	0.23550
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.293
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.5_2)
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	2.992	2.992
Number of reflections	42099
<I/σ(I)>	19.2
Completeness [%]	99.8	97.8
Redundancy	9.5	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	291	0.1 M sodium citrate, 0.2 M ammonium acetate, 25% (w/v) PEG 4000, 1% (v/v) ethylene glycol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K