3NOE

Crystal Structure of Dihydrodipicolinate synthase from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	292
Detector technology	IMAGE PLATE
Collection date	2009-09-28
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.541
Spacegroup name	P 21 21 21
Unit cell lengths	58.420, 81.870, 124.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.910 - 2.950
R-factor	0.202
Rwork	0.202
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yxc
RMSD bond length	0.008
RMSD bond angle	1.700
Data reduction software	AUTOMAR
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.910	3.100
High resolution limit [Å]	2.950	2.950
Number of reflections	12763
Completeness [%]	97.0	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	30% PEG 3350, 120mM MgCl2, 70mM Tris-HCl, 6% propylene glycol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K