3NNH
Crystal Structure of the CUGBP1 RRM1 with GUUGUUUUGUUU RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.180, 62.074, 122.052 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.596 - 2.750 |
| R-factor | 0.1909 |
| Rwork | 0.183 |
| R-free | 0.26280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nmr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.031 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.114 | 0.376 |
| Number of reflections | 12357 | |
| <I/σ(I)> | 13.1 | 4.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M potassium sodium tartrate, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
