3NMW
Crystal structure of armadillo repeats domain of APC
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-25 |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.389, 52.682, 63.451 |
| Unit cell angles | 90.31, 89.95, 95.16 |
Refinement procedure
| Resolution | 50.000 - 1.600 |
| R-factor | 0.16959 |
| Rwork | 0.168 |
| R-free | 0.20112 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.417 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
| Rmerge | 0.075 | 0.060 | 0.342 |
| Number of reflections | 165928 | ||
| <I/σ(I)> | 13.3 | ||
| Completeness [%] | 95.1 | 96.7 | 83.1 |
| Redundancy | 1.9 | 2 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 287 | 25% PEG 3350, 180mM ammonium sulfate, pH 8.0, vapor diffusion, sitting drop, temperature 287K |
