3NL6
The Crystal Structure of Candida glabrata THI6, a Bifunctional Enzyme involved in Thiamin Biosyhthesis of Eukaryotes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97920 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 163.173, 153.528, 109.618 |
Unit cell angles | 90.00, 117.99, 90.00 |
Refinement procedure
Resolution | 39.000 - 2.612 |
R-factor | 0.2231 |
Rwork | 0.221 |
R-free | 0.27140 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.195 |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.000 |
High resolution limit [Å] | 2.600 |
Number of reflections | 69157 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 30% PEG400, 200 mM MgCl2, 100 mM HEPES, pH 7.5, vapor diffusion, hanging drop, temperature 295K |