3NL0
Mutant P44S M296I of Foot-and-mouth disease Virus RNA-dependent RNA polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 93.713, 93.713, 99.723 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.320 - 2.600 |
| R-factor | 0.22867 |
| Rwork | 0.226 |
| R-free | 0.27412 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wne |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.851 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.110 |
| Number of reflections | 15946 |
| <I/σ(I)> | 14.8 |
| Completeness [%] | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 35% PEG 4000, 0.2M sodium acetate, 0.1M sodium citrate, 4% butyrolactone , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
