3NKY

Structure of a mutant P44S of Foot-and-mouth disease Virus RNA-dependent RNA polymerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-04
Detector	ADSC QUANTUM 210
Wavelength(s)	0.933
Spacegroup name	P 41 21 2
Unit cell lengths	93.530, 93.530, 121.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.760 - 2.280
R-factor	0.24082
Rwork	0.240
R-free	0.26472
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u09
RMSD bond length	0.006
RMSD bond angle	0.856
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	REFMAC ((rigid body refinement))
Refinement software	REFMAC ((rigid body refinement))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.760
High resolution limit [Å]	2.280
Rmerge	0.076
Number of reflections	24188
<I/σ(I)>	16.6
Completeness [%]	96.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	35% PEG 4000, 0.2M sodium acetate, 0.1M sodium citrate, 4% butyrolactone, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K