3NKX
Impaired binding of 14-3-3 to Raf1 is linked to Noonan and LEOPARD syndrome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97809 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.410, 83.850, 111.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.760 - 2.400 |
| R-factor | 0.20622 |
| Rwork | 0.204 |
| R-free | 0.24733 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qjb |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.990 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.760 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.052 | 0.416 |
| Number of reflections | 27180 | |
| <I/σ(I)> | 4.02 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M (Sodium propionate, sodium cacodylate, BIS-TRIS propane), 27% PEG 1500, 2mM DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
