3NKV
Crystal structure of Rab1b covalently modified with AMP at Y77
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-02-05 |
| Detector | PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.970, 100.589, 45.071 |
| Unit cell angles | 90.00, 102.43, 90.00 |
Refinement procedure
| Resolution | 19.800 - 1.700 |
| R-factor | 0.1515 |
| Rwork | 0.150 |
| R-free | 0.18686 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.053 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.053 | 0.313 |
| Number of reflections | 33392 | |
| <I/σ(I)> | 15.8 | 4.5 |
| Completeness [%] | 99.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 298 | 15% (v/v) isopropanol, 15% (w/v) PEG4000, 0.1 M imidazole, 10 mM BaCl2, pH 6.0, vapor diffusion, hanging drop, temperature 298K |
