3NKP
Crystal structure of mouse autotaxin in complex with 18:1-LPA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-16 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.523, 94.518, 75.503 |
Unit cell angles | 90.00, 94.55, 90.00 |
Refinement procedure
Resolution | 26.530 - 1.751 |
R-factor | 0.191 |
Rwork | 0.189 |
R-free | 0.22220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nkm |
RMSD bond length | 0.007 |
RMSD bond angle | 1.196 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.6_289) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.750 |
Number of reflections | 84526 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20-25% PEG 3350, 0.15mM NaCl, 0.5M KSCN, 0.2mM ZnSO4, 1% polyvinylpyrrolidone, 0.2mM 18:1-LPA, VAPOR DIFFUSION, SITTING DROP, temperature 293K |