3NK4

Crystal structure of full-length sperm receptor ZP3 at 2.0 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9757
Spacegroup name	P 41 21 2
Unit cell lengths	97.700, 97.700, 256.419
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.344 - 2.000
R-factor	0.2092
Rwork	0.208
R-free	0.22580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3D4G: PDB ENTRY 3D4G: RESIDUES 372-449 + 459-473
RMSD bond length	0.010
RMSD bond angle	1.224
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.900	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.092
Number of reflections	84686
<I/σ(I)>	12.6	2
Completeness [%]	99.9	100
Redundancy	13.3	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	25 MG/ML PROTEIN IN 0.1 M SODIUM CITRATE, 0.01 M TRIS-HCL, 5% PEG6000, 0.05 M SODIUM CHLORIDE, SAMPLE TO RESERVOIR RATIO IN DROP: 1:1 (DROP), pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K