3NK3

Crystal structure of full-length sperm receptor ZP3 at 2.6 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-28
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 41 21 2
Unit cell lengths	98.385, 98.385, 257.369
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.318 - 2.600
R-factor	0.2202
Rwork	0.219
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3D4G: PDB ENTRY 3D4G: RESIDUES 372-449 + 459-473
RMSD bond length	0.011
RMSD bond angle	1.123
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.200	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.136
Number of reflections	37990
<I/σ(I)>	10.8	2
Completeness [%]	95.4	89.5
Redundancy	6.2	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	25 MG/ML PROTEIN IN 0.1 M SODIUM, CITRATE, 0.01 M TRIS-HCL, 4% PEG6000, 0.05 M SODIUM CHLORIDE, SAMPLE TO RESERVOIR RATIO IN DROP: 1:1 (DROP), pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K