3NGI
RB69 DNA Polymerase (Y567A) Ternary Complex with dTTP Opposite dG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.989, 120.336, 130.768 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.886 |
| R-factor | 0.19371 |
| Rwork | 0.192 |
| R-free | 0.22623 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nci |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.010 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.110 | 1.000 |
| Number of reflections | 95329 | |
| <I/σ(I)> | 12.4 | 1.04 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | micro-batch vapor-diffusion | 6.5 | 293 | 13%(w/v) PEG 350 monomethyl ether (MME), 150 mM CaCl2, 100 mM NaCacodylate (pH 6.5), micro-batch vapor-diffusion, temperature 293K |
