3NG2
Crystal structure of the RNF4 ring domain dimer
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 291 |
Detector technology | CCD |
Collection date | 2009-07-17 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.2829, 1.2833, 1.2523 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 58.487, 85.922, 22.277 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.730 - 1.800 |
R-factor | 0.19001 |
Rwork | 0.187 |
R-free | 0.24199 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.546 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.010 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.067 | 0.274 |
Number of reflections | 10936 | |
<I/σ(I)> | 16.1 | 6.7 |
Completeness [%] | 99.1 | 98.8 |
Redundancy | 6.6 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 291 | 0.2M lithium sulfate, 0.1M bis-Tris, 22% PEG 3350, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |