3NG0
Crystal Structure of Glutamine Synthetase from Synechocystis sp. PCC 6803
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 132.016, 132.016, 202.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.920 - 2.800 |
| R-factor | 0.2867 |
| Rwork | 0.285 |
| R-free | 0.31290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.160 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
| Rmerge | 0.213 | 0.157 | 0.424 |
| Number of reflections | 25802 | ||
| <I/σ(I)> | 4.1 | ||
| Completeness [%] | 98.0 | 93.4 | 99.2 |
| Redundancy | 6.7 | 6.3 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 0.1M sodium acetate,0.1M calcium acetate, 10% PEG 4000, pH 4.0, vapor diffusion, hanging drop, temperature 298K |
