3NFF

Crystal structure of extended Dimerization module of RNA polymerase I subcomplex A49/A34.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	70
Detector technology	CCD
Collection date	2008-12-04
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9330
Spacegroup name	C 2 2 21
Unit cell lengths	108.930, 221.770, 129.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.100 - 3.240
R-factor	0.2904
Rwork	0.287
R-free	0.36080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Crystal structure of Dimerization module of RNA polymerase I subcomplex A49/A34.5 (SG P 21)
RMSD bond length	0.010
RMSD bond angle	1.250
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.9.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	3.380
High resolution limit [Å]	3.240	3.240
Rmerge	0.207	0.792
Number of reflections	25171
<I/σ(I)>	5.58	3.7
Completeness [%]	99.4	99.5
Redundancy	8	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	20% PEG 3350, 50 mM Tris pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K