3NEM

Aspartyl-tRNA synthetase complexed with aspartyl adenylate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1997-03-12
Detector	MAR scanner 345 mm plate
Spacegroup name	P 21 21 2
Unit cell lengths	123.220, 124.870, 86.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.580 - 1.890
Rwork	0.188
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1asz
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.630
High resolution limit [Å]	1.890
Number of reflections	103073
Completeness [%]	95.7
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1			297	peg and ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 297K