3NEK
Crystal structure of a nitrogen repressor-like protein MJ0159 from Methanococcus jannaschii
Replaces: 2QYXExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 80.009, 80.009, 116.421 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.150 - 2.500 |
| R-factor | 0.247 |
| Rwork | 0.246 |
| R-free | 0.27700 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.116 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.075 | 0.590 |
| Number of reflections | 15322 | |
| <I/σ(I)> | 7.1 | 1 |
| Completeness [%] | 99.2 | 98.8 |
| Redundancy | 4.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 100mM sodium MES pH 7.0, 40% MPD, vapor diffusion, temperature 294K |
