3NBZ
Crystal structure of the HIV-1 Rev NES-CRM1-RanGTP nuclear export complex (crystal I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.87260 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.660, 224.617, 164.021 |
| Unit cell angles | 90.00, 100.82, 90.00 |
Refinement procedure
| Resolution | 38.897 - 2.800 |
| R-factor | 0.2291 |
| Rwork | 0.226 |
| R-free | 0.28500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3gjx |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.938 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.128 |
| Rmeas | 0.152 |
| Total number of observations | 333225 |
| Number of reflections | 114008 |
| <I/σ(I)> | 7.05 |
| Completeness [%] | 90.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 289 | 0.1 M Tris/HCl, 16% (w/v) PEG 1000, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
