3NB0
Glucose-6-Phosphate activated form of Yeast Glycogen Synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 192.739, 206.982, 205.799 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.229 - 2.406 |
| R-factor | 0.213 |
| Rwork | 0.211 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3naz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.737 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.087 | 0.552 |
| Number of reflections | 157186 | |
| <I/σ(I)> | 15.5 | |
| Completeness [%] | 98.5 | 79.1 |
| Redundancy | 4.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 0.1M Bis-Tris pH 6.2, 25% PEG 300, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
