3NAF

Structure of the Intracellular Gating Ring from the Human High-conductance Ca2+ gated K+ Channel (BK Channel)

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	200
Detector technology	CCD
Collection date	2010-03-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	I 4 2 2
Unit cell lengths	134.395, 134.395, 231.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.483 - 3.100
R-factor	0.2429
Rwork	0.238
R-free	0.28890
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.709
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.160
High resolution limit [Å]	3.100	3.100
Rmerge	0.063	0.529
Number of reflections	19761
<I/σ(I)>	30	2.2
Completeness [%]	96.2	80
Redundancy	8.8	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	3.5M sodium formate, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	3.5M sodium formate, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K