3NA6

Crystal structure of a succinylglutamate desuccinylase (TM1040_2694) from SILICIBACTER SP. TM1040 at 2.00 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-11
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97922,0.97905
Spacegroup name	P 63 2 2
Unit cell lengths	99.773, 99.773, 137.179
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.487 - 2.000
R-factor	0.163
Rwork	0.161
R-free	0.19500
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.513
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.487	29.487	2.070
High resolution limit [Å]	2.000	4.300	2.000
Rmerge	0.157	0.013	0.013
Number of reflections	27876	5232	4903
<I/σ(I)>	11.56	39.1	1.8
Completeness [%]	99.5	99.6	96.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.2000M NaCl, 30.0000% PEG-3000, 0.1M TRIS pH 7.0, 0.006 M Calcium Chloride, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K